Digital inline holography dihm is an imaging modality within the fast. The presented 3dprintable portable opensource platform represents a fullyopen design, lowcost modular and versatile imagingsolution for use in high and lowresourceareasoftheworld. Our lensless system consists solely of a diffuser placed in front of a standard image sensor. A 3d printable platform for lensless holographic cellular imaging with open accessible software solutions has been developed delivering spatial resolutions of 1. Basic lensless imaging for lowcost microscopy hackaday. Holographic imaging and iterative phase optimization. We experimentally built and tested a lensless imaging system where a dnn was trained to recover phase objects. Pdf lensless multispectral digital inline holographic microscope. Fast 3d imaging with lensless holographic endoscopy employing coherent fiber bundles, by juergen w. Digital holography systems that employ shadow imaging projection of 2d. Ozcan, partiallycoherent lensless holographic microscopy for automated semen analysis on a chip. Spotlight including stock price, stock chart, company news, key statistics, fundamentals and company profile. This incoherent holographic imaging and diagnostic modality has orders of. Lensless microscopes, just like holographic ones, have several advantages.
The software also supports 3d imaging from the reconstructed stack of. Holographic pixel superresolution in portable lensless on. The innovative new technology takes data from ct and mri scans and transforms it into 3d holographic images using 3d glasses, a laser stylus, and a special display. Color imaging in lensless digital holographic microscopy. Digital holographic microscopy dhm is a technique that uses optical interference patterns to record a threedimensional optical field for imaging, sensing, and microscopy applications. A new lensless imaging system is built to record holographic videos of soil particles flowing through microscale flow assays. After sample cleaning using 50% optiprep by volume, a 1 ml solution of the interface layer was loaded into a syringe. Introduction digital inline holography dihm is an imaging modality within the fast evolving.
We propose a method for acquiring color images with a lensless inline holographic microscope lihm using sunlight illumination, which is suitable for weakly scattered amplitude objects. Tiny lensless endoscope captures 3d images of objects. The lensless cellmicroscope combines holographic imaging of cells with. The dispensing needle of the syringe was connected to the input port of the microfluidic flow chip. The syringe pump was turned on at a flow rate of 1 ml per hour. Holoscopy makes ultrafast lensless imaging of scattering tissues possible. Automated image analysis systems to quantify physical and. Here, we demonstrate for the first time to our knowledge that deep neural networks dnns can be trained to solve endtoend inverse problems in computational imaging. For weaklyscattered amplitude object, we show that the.
Stock analysis for phase holographic imaging phi ab phi. Improving lensless computational microscopy technology. Partially coherent lensless holographic microscopy with. The principle of holographic imaging invented many decades ago has significantly advanced since the original digital inline holographic experiment 2,40. Biomedical optical phase microscopy and nanoscopy, elsevier published in 20 isbn. Dlhm is a lensless methodology of imaging in two stages. A multimodal novel lensless microscopy technology for. Deep learning has been proven to yield reliably generalizable solutions to numerous classification and decision tasks.
Digital lensless holographic microscopy and applications. Project basic lensless imaging for lowcost microscopy. Spotlight stock quote phase holographic imaging phi. In this paper, an irregular displacementbased lensless widefield microscopy imaging platform is presented by combining digital inline holography and computational pixel superresolution using. The aim of the present work is to develop digital dynamic holography as a metrological tool for both transmission and reflection applications.
By combining digital holography with optical coherence tomography oct in a process known as holoscopy, 3d parallel image acquisition with extremely high data throughput at high imaging speeds is possible due to the lack of moving elements. Dear colleagues, recent advancements in the semiconductor industry have inspired a revolution in compact sensors and computational platforms, creating a paradigm shift from lensbased imaging and sensing to computational imaging and sensing. Science foundation nsf cbet division biophotonics program, the nsf emerging frontiers in. Compact lensless digital holography infoscience epfl. We demonstrate a compact and easytobuild computational camera for singleshot 3d imaging. Software algorithms are written to automatically identify the nematode eggs from low resolution holographic videos or images captured from a scanner. Osa color lensless inline holographic microscope with.
Interfacing the device with koala software from lynceetec is considered in. A lensless inline digital holographic microscopy system is proposed and its performance for dynamic 3d imaging and micromeasurements is. A lensless microscope capable of generating holograms of biotargets with. Micromanager is an open source platform for controlling microscope hardware, automating image acquisition, and generating metadata about how images are acquired. In a regular optical microscope, the seen image is a highly magnified photographic image. In this paper, an irregular displacementbased lensless widefield microscopy imaging platform is presented by combining digital inline holography and. In the literature, there is a significant number of approaches developed upon the traditional holography principle, where a splitter is commonly used to separate object and reference beams 41. But, if the object of study is transparent like, for instance, many living cells, photographic images cant be captured unless one uses special coloring agents.
Judy yee, professor and vice chair of radiology and biomedical imaging and chief of radiology at sfvamc, described echopixels true 3d software. In the color lihm, the sample is illuminated by sunlight, and the inline hologram is recorded by a color cmos imaging sensor located behind the sample. The ozcan research group refereed journal publications. Lensless microscopy of cell cultures 2014 wiley analytical science. In this platform, lensfree digital holograms of the microobjects on the chip are recorded at different illumination angles using partially coherent illumination.
The principles and some applications of the digital lensless holographic. Pedrini, w osten 2005 application of shortcoherence digital holography in microscopy. An introduction to lensless digital holographic microscopy. Tiziani 2002 shortcoherence digital microcopy by use of lensless holographic imaging system, appl. Both printer and reconstruction software source codes are open. After traveling a distance r, the phase of the reference beam in the plane of the. Depth resolved imaging using multiangle lensless holography. Portable lensless widefield microscopy imaging platform. After the capture of the multiangle lensfree holographic images of the sample volume as illustrated in fig.
Using holography instead of optical focusing enables to build a costefficient, robust and compact microscope. Lensless singleexposure 3d imaging nick antipa, grace kuo, reinhard heckel, ben mildenhall, emrah bostan, ren ng, and laura waller. Osa multiangle lensless digital holography for depth. The details of the depth resolved imaging process using multiangle lensfree holography is summarized in fig. Czarske, elias scharf, and robert kuschmierz, will be presented monday, 16. Portable lensless widefield microscopy imaging platform based on. A 3d printable platform for lensless holographic cellular imaging with open. Continuous livecell culture imaging and singlecell tracking by computational lensfree led microscopy, sensors 2019. Lensless inline digital holography for dynamic applications. The cooling of the cmos camera defines the local temperature of the integrated cell culture chamber. Computational microscopy, sensing and diagnostics for telemedicine and global health applications.
Holograms can also be used for integral imagingbased 3d display, lensless projection, and lidar beam steering. Holopy digital holography and light scattering tools in python. In conclusion, we reported a lensless holographic superresolution microscope which is quite robust, easy to use, portable 95 g and costeffective, achieving a resolution of software platform converts existing 2d medical imaging data such as mri and. Osa lensless computational imaging through deep learning. Lensless inline dhm is the simplest arrangement, requiring no lenses, no mirrors, and typically only a light source, sample, and a digital imager chip. In biomedical imaging research, it serves as an incredibly important tool because it is free and open source, which means that scientists can benefit from the contributions of others to the software without paying costly licensing fees. The lensless cellmicroscope combines holographic imaging of cells with a thermoelectric cooling system and automated cell segmentation for live cell imaging inside the incubator.
226 421 82 455 1019 1024 819 387 30 1166 1342 1377 1423 401 1410 964 767 905 1572 1339 752 849 1309 168 572 682 231 1398 553 116 1490 560 278 376 20 877 1141 410 292